In practice, plants containing the PAT protein have been widely cultivated in the United States of America and Canada for almost a decade, without any reports of adverse effects when used in human and animal nutrition. Thus, the inclusion of PAT protein in human and animal food does not cause harm. The action of this protein is well known and there is no evidence in the literature that it has a biocidal action against non-target organisms. The trait conferred by the bar gene - herbicide tolerance - is recognized as not being able to endow the receptor genotypes with any adaptive advantage outside agricultural areas, since outside these areas, the potential wild receptor genotypes are not affected by the selective pressure of the glufosinate ammonium herbicide and, therefore, the eventual pollination of these genotypes would not result in gene introgression. It is extremely unlikely that the bar transgene from Event LLCotton25 will be transferred to weeds making them more invasive. The agronomic tests carried out attached to the process and the reports of the planned releases in the environment did not identify differences between the LLCotton25 cotton plant and its isoline in terms of susceptibility to diseases and pests. The possibility of the herbaceous cotton plant LLCotton25 becoming a weed is considered negligible. The analysis of the biochemical composition of LibertyLink Cotton demonstrates that the LLCotton25 event presents substantial equivalence to non-genetically modified varieties, strongly suggesting that the event in question does not present undesirable pleiotropic effects. None of the phenotypic characteristics of the genetically modified cotton plants suffered any alteration due to the insertion and expression of the bar gene in relation to the unmodified cultivars.